Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization.

AUTOR(ES)

Czichos, J

RESUMO

Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very simple one step procedure. Some of such DNA-protein conjugates have been used as probes for hybridization experiments. DNA-protein A probes were found to be most useful.

Documentos Relacionados

• Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation.
• Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
• Use of biotinylated DNA probes in colony hybridization.
• Protein–DNA computation by stochastic assembly cascade
• Protein–DNA footprinting by endcapped duplex oligodeoxyribonucleotides