Protein-free cell culture on an artificial substrate with covalently immobilized insulin.
AUTOR(ES)
Ito, Y
RESUMO
Insulin was immobilized on a surface-hydrolyzed poly(methyl methacrylate) film. Chinese hamster ovary cells overexpressing human insulin receptors were cultured on the film in the absence of serum or soluble proteins. Small amounts of immobilized insulin (1-10% of the required amount of free insulin) were sufficient to stimulate cell proliferation. In addition, the maximal mitogenic effect of immobilized insulin was greater than that of free insulin. Immobilized insulin activated the insulin receptor and downstream signaling proteins, and this activation persisted for longer periods than that obtained with free insulin, probably explaining the greater mitogenic effect of the immobilized insulin. Finally the immobilized-insulin film was usable repeatedly without marked loss of activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39656Documentos Relacionados
- CYTOLOGICAL PREPARATIONS FROM PROTEIN-FREE FLUIDS
- Cytological Preparations from Protein-free Fluids
- Protein-free parallel triple-stranded DNA complex formation
- Protein-free and low-protein media for the cultivation of Leptospira.
- Flickering fusion pores comparable with initial exocytotic pores occur in protein-free phospholipid bilayers
