Protein Synthesis in Escherichia coli with Mischarged tRNA
AUTOR(ES)
Min, Bokkee
FONTE
American Society for Microbiology
RESUMO
Two types of aspartyl-tRNA synthetase exist: the discriminating enzyme (D-AspRS) forms only Asp-tRNAAsp, while the nondiscriminating one (ND-AspRS) also synthesizes Asp-tRNAAsn, a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). On the basis of the E. coli trpA34 missense mutant transformed with heterologous ND-aspS genes, we developed a system with which to measure the in vivo formation of Asp-tRNAAsn and its acceptance by elongation factor EF-Tu. While large amounts of Asp-tRNAAsn are detrimental to E. coli, smaller amounts support protein synthesis and allow the formation of up to 38% of the wild-type level of missense-suppressed tryptophan synthetase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=156233Documentos Relacionados
- Interaction of Escherichia coli glutaminyl-tRNA synthesis with noncognate tRNA's.
- LEUCINE tRNA AND CESSATION OF Escherichia coli PROTEIN SYNTHESIS UPON PHAGE T2 INFECTION*
- Temperature sensitive mutants of Escherichia coli for tRNA synthesis
- Eukaryotic selenocysteine inserting tRNA species support selenoprotein synthesis in Escherichia coli.
- Level of rRNA, not tRNA, synthesis controls transcription of rRNA and tRNA operons in Escherichia coli.