Proteinase C (carboxypeptidase Y) mutant of yeast.
AUTOR(ES)
Wolf, D H
RESUMO
A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of proteinase C does not affect mitotic growth and has no obvious effect on the formation of viable ascospores or meiotic segregation. The mutant grows on peptides known to be cleaved by proteinase C in vitro. This finding is consistent with the idea that other enzymes exist in vivo with overlapping substrate specificities.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235840Documentos Relacionados
- Maturation of vacuolar (lysosomal) enzymes in yeast: proteinase yscA and proteinase yscB are catalysts of the processing and activation event of carboxypeptidase yscY.
- Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
- Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated.
- Isolation of a peptide transport-deficient mutant of yeast.
- Isolation and Characterization of the Carboxypeptidase Y Inhibitor from Yeast
