Proton Nuclear Magnetic Resonance Studies of Ribonuclease A in H2O
AUTOR(ES)
Patel, Dinshaw J.
RESUMO
A resonance (designated a) due to an exchangeable proton titrates (pKa = 6.1) between 11.5 and 13 ppm in the nuclear magnetic resonance spectrum of RNase A-0.2 M NaCl in H2O at 20°. Comparison with models has permitted assignment to a ring-nitrogen proton of histidine in slow exchange with solvent H2O. The pH and temperature-dependent line-width changes of resonance a are analyzed in terms of an exchange between histidine and protonated histidine, without the necessity to invoke any exchange processes associated with protein conformational changes. Several other resonances due to exchangeable protons are observed between 10 and 15 ppm in the nuclear magnetic resonance spectrum of RNase A in H2O.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426515Documentos Relacionados
- Proton Nuclear Magnetic Resonance Studies of Myoglobin in H2O
- Carbon-13 Relaxation and Proton Nuclear Magnetic Resonance Studies of Prostaglandin F2α
- Proton magnetic resonance studies of human cyanomethemoglobin.
- Proton magnetic resonance studies of 2'-,3'-, and 5'-deoxyadenosine conformations in solution.
- Nuclear magnetic resonance study of proton conductor HPb2Nb3O10. nH2O.