PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry

AUTOR(ES)

Herrmann, Lynn M.

FONTE

American Society for Microbiology

RESUMO

Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% ± 0.28% of 3 × 106 DRLN cells, but were not detected in 3 × 106 PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 × 106 PBLs.

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