Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells.
AUTOR(ES)
Redfield, D C
RESUMO
Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=351582Documentos Relacionados
- Polysome-associated proteins in herpes simplex virus-infected cells.
- Ontogeny of murine cellular cytotoxicity to herpes simplex virus-infected cells.
- Murine cellular cytotoxicity to syngeneic and xenogeneic herpes simplex virus-infected cells.
- Herpes simplex virus-infected cells disarm killer lymphocytes.
- Characterization of polysome-associated RNA from influenza virus-infected cells.