Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633
AUTOR(ES)
Hermes, H. F. M.
RESUMO
An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and α-H amino acid amides, e.g., l-phenylglycine amide.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=195905Documentos Relacionados
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