Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum.
AUTOR(ES)
Pich, A
RESUMO
The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Escherichia coli RNA polymerase identified the 46,000-Da subunit as an immunologically and probably functionally related protein. The other three subunits of 128,000, 117,000, and 42,000 Da are tentatively analogous to the beta, beta', and alpha subunits, respectively, of other eubacterial RNA polymerases. The RNA polymerase activity was completely dependent on Mg2+, nucleoside triphosphates, and a DNA template. The presence of Mg2+ or Mn2+ in buffers used for purification or storage caused irreversible inactivation of the RNA polymerase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207749Documentos Relacionados
- Purification and partial characterization of the DNA-dependent RNA polymerase from Rickettsia prowazekii.
- Purification of the DNA-dependent RNA polymerase from the myxobacterium Stigmatella aurantiaca.
- Purification and Characterization of DNA-dependent RNA Polymerases from Cauliflower Nuclei 1
- DNA-Dependent RNA Polymerase from Yeast Mitochondria
- Purification and Subunit Structure of DNA-dependent RNA Polymerase III from Wheat Germ 1
