Purification and Characterization of the L Component of Streptococcus zymogenes Lysin
AUTOR(ES)
Granato, Paul A.
RESUMO
By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the L component of the group D lysin produced by Streptococcus zymogenes strains has been purified to a state of homogeneity as determined by the techniques of disc-gel electrophoresis at pH 9.3 and 4.3 and isoelectric focusing. The L component was found to be a protein possessing a molecular weight of 11,000 with a slight net negative charge at physiological pH.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247144Documentos Relacionados
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