Purification and functional characterization of a cellular transcription factor that binds to an enhancer element within the adenovirus early EIIa promoter.
AUTOR(ES)
Jalinot, P
RESUMO
The adenovirus EIa-inducible early EIIa (EIIaE) promoter is comprised of several sequence elements essential for constitutive and induced expression. We report here the purification of the host-cell factor that interacts with the major upstream element of this promoter, extending between positions -90 and -70 with respect to the main EIIaE cap site and exhibiting enhancer properties. The purified factor, which corresponds to a 40- to 43-kDa polypeptide, specifically binds to its recognition site and stimulates EIIaE promoter activity when added to an in vitro transcription system, reconstituted from purified factors and RNA polymerase. The implication of this factor in the control of the other adenovirus early genes is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=280021Documentos Relacionados
- EIa-mediated stimulation of the adenovirus EIII promoter involves an enhancer element within the nearby EIIa promoter.
- Specific cellular proteins bind to critical promoter sequences of the adenovirus early EIIa promoter.
- Negative regulatory sequences in the EIa-inducible enhancer of the adenovirus-2 early EIIa promoter.
- Cellular transcription factor binds to adenovirus early region promoters and to a cyclic AMP response element.
- Two distinct cellular proteins interact with the EIa-responsive element of an adenovirus early promoter.