Purification and Properties of an Extracellular Agarase from Alteromonas sp. Strain C-1

AUTOR(ES)

Leon, Oscar

RESUMO

A marine bacterial strain isolated from the Bay of San Vicente, Chile, was identified as Alteromonas sp. strain C-1. In the presence of agar, this strain produced high levels of an extracellular agarase. The production of agarase was repressed by glucose, with a parallel decrease in bacterial growth. The enzyme was purified to homogeneity by anion-exchange chromatography and gel filtration, with an overall yield of 45%. The enzyme has a molecular weight of 52,000, is salt sensitive, and hydrolyzes agar, yielding neoagarotetraose as the main product, with an optimum pH of about 6.5.

Documentos Relacionados

• Purification and properties of formate dehydrogenase from Moraxella sp. strain C-1.
• Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107.
• Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.
• Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138.
• Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646.