Purification and Subunit Structure of DNA-dependent RNA Polymerase III from Wheat Germ 1
AUTOR(ES)
Jendrisak, Jerry
RESUMO
A rapid and simple, large-scale method for the purification of DNA-dependent RNA polymerase III (EC 2.7.7.6) from wheat germ is presented. The method involves enzyme extraction at low ionic strength, polyethyleneimine fractionation, (NH4)2SO4 precipitation, and chromatography on DEAE-Sepharose CL-6B, DEAE-cellulose, and heparin agarose. Milligram quantities of highly purified enzyme can be obtained from kilogram quantities of starting material in 2 to 3 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates that RNA polymerase III contains 14 subunits with molecular weights of: 150,000; 130,000; 94,000; 55,000; 38,000; 30,000; 28,000; 25,000; 24,500; 20,500; 20,000; 19,500; 17,800; and 17,000. Subunit structure comparison of wheat germ RNA polymerases I, II, and III indicates that all three enzymes may contain common subunits with molecular weights 20,000, 17,800, and 17,000. In addition, RNA polymerases II and III may contain a common subunit with a molecular weight of 25,000, and RNA polymerases I and III may contain a common subunit with a molecular weight of 38,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=425701Documentos Relacionados
- DNA-dependent RNA polymerases from Artemia salina. Subunit structure of polymerase II.
- RNA Polymerases of Maize. Purification and Molecular Structure of DNA-dependent RNA Polymerase II*
- Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum.
- Purification and partial characterization of the DNA-dependent RNA polymerase from Rickettsia prowazekii.
- Purification of the DNA-dependent RNA polymerase from the myxobacterium Stigmatella aurantiaca.