Purification of cDNA complementary to sea urchin histone mRNA.
AUTOR(ES)
Woods, D
RESUMO
Complementary DNA (cDNA) was transcribed from a polyadenylated sea urchin histone mRNA preparation isolated by density gradient centrifugation. By hybridization, this cDNA was shown to be extensively contaminated (85% of hybridizable cDNA) with DNA complementary to RNA derived from the large ribosomal subunit. Purification of a mRNA specific cDNA fraction was achieved by hybridization of purified rRNA to cDNA followed by fractionation on hydroxyapatite. After further purification to remove nonhybridizable cDNA our purified cDNA showed only 8% hybirdization to rRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342643Documentos Relacionados
- The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
- Molecular cloning of a cDNA sequence complementary to porphobilinogen deaminase mRNA from rat.
- Message-specific sequestration of maternal histone mRNA in the sea urchin egg.
- Mutually exclusive synthetic pathways for sea urchin mitochondrial rRNA and mRNA.
- Human apolipoprotein B: identification of cDNA clones and characterization of mRNA.
