Purification of DNA for bacterial productivity estimates.
AUTOR(ES)
Burnison, B K
RESUMO
[methyl-3H]thymidine-labeled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein by hydroxylapatite chromatography. The procedure was validated by monitoring increases in Escherichia coli cell count, A550, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolysis or volatile organic solvents.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=183345Documentos Relacionados
- Evaluation of swine diet formulation based on amino acid digestibility estimates.
- Reproductive fitness and frequency of new mutations in Becker muscular dystrophy: implications for genetic risk estimates.
- Choice of control population in studies of adverse reproductive effects of occupational exposures and its effect on risk estimates.
- An improved method for the purification of high molecular weight bacterial chromosomal DNA.
- A one-step procedure for the purification of high molecular weight bacterial chromosomal DNA.
