Purification of Intact Flagella from Escherichia coli and Bacillus subtilis

AUTOR(ES)

DePamphilis, M. L.

RESUMO

A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH4)2SO4 precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 μm of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis.

Documentos Relacionados

• Purification and Thermal Stability of Intact Bacillus subtilis Flagella
• Fine Structure and Isolation of the Hook-Basal Body Complex of Flagella from Escherichia coli and Bacillus subtilis
• Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli.
• Functional homology of chemotactic methylesterases from Bacillus subtilis and Escherichia coli.
• Bacillus subtilis β-1, 4-Endoglucanase Products from Intact and Truncated Genes Are Secreted into the Extracellular Medium by Escherichia coli†