Purification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography 1
AUTOR(ES)
Funkhouser, Edward A.
RESUMO
myo-Inositol 1-phosphate synthase (EC 5.5.1.4) is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold in a single step, about 9000-fold over-all, to a specific activity of 0.078 units per milligram of protein. This is an order of magnitude greater than previous purifications of the plant enzyme.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541925Documentos Relacionados
- The arabidopsis thaliana myo-inositol 1-phosphate synthase (EC 5.5.1.4).
- myo-Inositol 1-Phosphate Synthase Inhibition and Control of Uridine Diphosphate-d-glucuronic Acid Biosynthesis in Plants 12
- 1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana.
- myo-Inositol Metabolism in Lilium longiflorum Pollen: Uptake and Incorporation of myo-Inositol-2-3H 1
- Myo-inositol transport in Saccharomyces cerevisiae.