PURIFICATION OF STAPHYLOCOCCAL ALPHA-HEMOLYSIN
AUTOR(ES)
Madoff, Morton A.
RESUMO
Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914–918. 1962. — Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279374Documentos Relacionados
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