Quantification of the Flavonoid-Degrading Bacterium Eubacterium ramulus in Human Fecal Samples with a Species-Specific Oligonucleotide Hybridization Probe

AUTOR(ES)

Simmering, Rainer

FONTE

American Society for Microbiology

RESUMO

To investigate the occurrence of the flavonoid-degrading bacterium Eubacterium ramulus in the human intestinal tract, an oligonucleotide probe designated S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacterial strains representing the dominant described human fecal flora. Application of S-S-E.ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence of E. ramulus in each individual tested in numbers from 4.4 × 107 to 2.0 × 109 cells/g of fecal dry mass. Six fecal E. ramulus isolates were recognized by S-S-E.ram-0997-a-A-18 but exhibited different band patterns when analyzed by randomly amplified polymorphic DNA.

Documentos Relacionados

• Quantification of Different Eubacterium spp. in Human Fecal Samples with Species-Specific 16S rRNA-Targeted Oligonucleotide Probes
• Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.
• Species-specific identification of Candida krusei by hybridization with the CkF1,2 DNA probe.
• Preliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia.
• Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization