Pinhati, Renata Romanholi, Polonini, Hudson Caetano, Brandão, Marcos Antônio Fernandes, Raposo, Nádia Rezende Barbosa, Corchs, Felipe, Gattaz, Wagner Farid
2012A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.