Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay.
AUTOR(ES)
Panet, A
RESUMO
A radioimmunoassay was developed that can detect and quantitate 3 ng or more of the avian RNA tumor virus reverse transcriptase. The assay detected no antigenic sites in Rous sarcoma virus alpha virions or in virions of a murine RNA tumor virus. About 70 molecules of reverse transcriptase were found per virion of avian myleloblastosis virus with this assay or with an assay based on antibody inhibition of enzymatic activity. The assay detected about 270 ng of enzyme per mg of cell protein in virus-producing cells; uninfected cells had much less antigenic material but contained some determinants able to displace radioactive antigen. No additional antigenic determinants on reverse transcriptase could be detected that were not found on the separated alpha subunit of the enzyme. Although sevenfold less sensitive than enzymatic activity as a measure of reverse transcriptase, the radioimmunoassay can detect antigen using small amounts of protein and in the presence of inhibtors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354643Documentos Relacionados
- Detection of human immunodeficiency virus by reverse transcriptase assay, antigen capture assay, and radioimmunoassay.
- Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.
- Studies on the Reverse Transcriptase of RNA Tumor Viruses. Structural Relatedness of Two Subunits of Avian RNA Tumor Viruses*
- RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.
- Reverse transcriptase of RNA tumor viruses. V. In vitro proteolysis of reverse transcriptase from avian myeloblastosis virus and isolation of a polypeptide manifesting only RNase H activity.
