Quantitative Analysis of the Acute and Long-Term CD4+ T-Cell Response to a Persistent Gammaherpesvirus
AUTOR(ES)
Christensen, Jan P.
FONTE
American Society for Microbiology
RESUMO
The murine gammaherpesvirus 68 (MHV-68) replicates in respiratory epithelial cells, where it establishes a persistent, latent infection limited predominantly to B lymphocytes. The virus-specific CD4+ T-cell response in C57BL/6 mice challenged intranasally with MHV-68 is detected first in the mediastinal lymph nodes and then in the cervical lymph nodes and the spleen. The numbers of MHV-68-specific CD4+ T cells generated in congenic mice homozygous for disruption of the β2-microglobulin gene tended to be higher, indicating that the absence of the CD8+ set in this group resulted in a compensatory response. The peak frequency within the splenic CD4+ T-cell population may reach 1:50 in the acute response; it then drops to 1:400 to 1:500 within 4 months and stays at that level in the very long term. Sorting for L-selectin (CD62L) expression established that all virus-specific CD4+ T cells were initially CD62Llow, with >80% maintaining that phenotype for the next 14 months. The overall conclusion is that MHV-68-specific CD4+ T cells remain activated (CD62Llow) and at a stable frequency in the face of persistent infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=104208Documentos Relacionados
- Analysis of Virus-Specific CD4+ T Cells during Long-Term Gammaherpesvirus Infection
- CD4+ T Cells and Gamma Interferon in the Long-Term Control of Persistent Friend Retrovirus Infection
- Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras
- Quantitative Analysis of Long-Term Virus-Specific CD8+-T-Cell Memory in Mice Challenged with Unrelated Pathogens
- Development and characterization of allospecific long-term human cytolytic T-cell lines.