Quantitative Aspects of Exchangeable Calcium in Spores of Bacillus megaterium

AUTOR(ES)

Rode, L. J.

RESUMO

Rode, L. J. (The University of Texas, Austin), and J. W. Foster. Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium. J. Bacteriol. 91:1589–1593. 1966.—More than 90% of the calcium in Ca45-labeled native spores was released from the cells during germination. Some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to pH 4 with HNO3. Ca, Mg, Na, Si, and Fe were displaced from the spores by H+. The adsorption of Ca45 by H-spores and its subsequent release were studied under a variety of conditions. The isolated “coat fraction” of spores adsorbed substantial amounts of Ca45. Release of the adsorbed Ca was achieved with various reagents.

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