Quantitative Immunocapture PCR Assay for Detection of Campylobacter jejuni in Foods

AUTOR(ES)

Waller, David F.

FONTE

American Society for Microbiology

RESUMO

The rapid detection of food-borne bacterial pathogens as part of a quality control program is necessary for the maintenance of a safe food supply. In this report, we present our findings for an immunocapture PCR method for the detection of Campylobacter jejuni in foods. The method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h. Assay results are quantitative, and one cell in a milliliter sample can be detected. Application of the method to spiked milk samples and chicken skin washes did not affect the sensitivity of the assay.

Documentos Relacionados

• A Real-Time PCR Assay for the Detection of Campylobacter jejuni in Foods after Enrichment Culture
• Application of the 5′-Nuclease PCR Assay in Evaluation and Development of Methods for Quantitative Detection of Campylobacter jejuni
• Detection of Campylobacter jejuni and Campylobacter coli in Environmental Waters by PCR Enzyme-Linked Immunosorbent Assay
• Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples
• Detection of Small Numbers of Campylobacter jejuni and Campylobacter coli Cells in Environmental Water, Sewage, and Food Samples by a Seminested PCR Assay