Quantitative Radioautography of Sugar Transport in Intestinal Biopsies from Normal Humans and a Patient with Glucose-Galactose Malabsorption

AUTOR(ES)

Stirling, Charles E.

RESUMO

Both galactose accumulation and phlorizin binding by columnar epithelial cells have been investigated in vitro with a recently developed technique for high-resolution, plastic-section radioautography which is particularly suited to small quantities of biopsy tissue. Grain density analysis of the radioautographs provides definitive support for the view that the cellular mechanisms underlying glucose-galactose absorption in laboratory animals are fully applicable to the small intestine of man. Even the number of sugar carriers at the microvillar membrane appears similar and the major quantitative difference, lower affinity for phlorizin in man, correlates with the finding that phlorizin is also a less potent inhibitor of uphill, galactose transport at the microvilli. In addition, radioautographs of biopsies taken 2 yr apart from a patient with glucose-galactose malabsorption provide evidence that the cellular defect in this inborn error of transport is a persistent reduction in the number of functioning sugar carriers at the microvillar membrane.

Documentos Relacionados

• Renal and intestinal hexose transport in familial glucose-galactose malabsorption
• Cloning and DNA Sequence Analysis of an Immunogenic Glucose-Galactose MglB Lipoprotein Homologue from Brachyspira pilosicoli, the Agent of Colonic Spirochetosis†
• Long-term effect of insulin on glucose transport and insulin binding in cultured adipocytes from normal and obese humans with and without non-insulin-dependent diabetes.
• Transport and Metabolism of Lactose, Glucose, and Galactose in Homofermentative Lactobacilli
• D-galactose transport in rat intestinal brush border membrane vesicles studied with a molecular-sieve technique.