Quantitative Surface-Enhanced Raman for Gene Expression Estimation
AUTOR(ES)
Sun, Lan
FONTE
The Biophysical Society
RESUMO
We demonstrate for the first time, to our knowledge, a unique gene expression assay by surface-enhanced Raman scattering (SERS) using nonfluorescent Raman labels to quantify gene expression at the resolution of alternative splicing using RNA extracted from cancer cells without any amplification steps. Our approach capitalizes on the inherent plasmon-phonon mode of SERS substrates as a self-referencing standard for the detection and quantification of genetic materials. A strategy integrating S1 nuclease digestion with SERS detection was developed to quantify the expression levels of splice junction Δ(9,10), a segment of the breast cancer susceptibility gene 1 (BRCA1) from MCF-7 and MDA-MB-231 cells. Quantification results were cross-validated using two Raman tags and qualitatively confirmed by RT-PCR. Our methodology based on SERS technology provides reliable gene expression data with high sensitivity, bypassing the intricacies involved in fabricating a consistent SERS substrate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2711453Documentos Relacionados
- Surface-enhanced Raman scattering on tunable plasmonic nanoparticle substrates
- Interplay between Near-Field Properties and Au Nanorod Cluster Structure: Extending Hot Spots for Surface-Enhanced Raman Scattering
- Spectroscopic characterization of formylferrocene thiosemicarbazone (TFF) by SERS (Surface-Enhanced Raman Scattering) and Resonance Raman techniques
- Electrooxidation of ethanol on Pt and PtRu surfaces investigated by ATR surface-enhanced infrared absorption spectroscopy
- Estudos SERS (Surface-Enhanced Raman Scattering) em meio de Líquido Iônico BMIPF6 usando eletrodo de prata.