Raman diagnosis of nucleic acid structure: sugar-puckering and glycosidic conformation in the guanosine moiety.

AUTOR(ES)

Nishimura, Y

RESUMO

Observations of Raman spectra of various nucleic acids indicate that the guanine ring breathing frequency is sensitive to the internal rotation angle around the glycosidic bond and to the conformation of the five-membered ring of the ribose residue that is directly connected with the guanine residue in question. It is found that 682 cm-1 for C2'-endo-anti, at 665 cm-1 for C3'-endo-anti, and at 625 cm-1 for C3'-endo-syn. A DNA octamer d(GpGpApApTpTpCpC) shows, in its aqueous solution, a broad Raman band at 680 cm-1 with a tail at 670 cm-1. This fact suggests that the guanosine residues in this oligomer take primarily C2'-endo-anti conformation but an appreciable amount of fluctuation of the ribose ring structure towards C3'-endo is involved.

Documentos Relacionados

• Determination of the Backbone Structure of Nucleic Acids and Nucleic Acid Oligomers by Laser Raman Scattering
• Structure-spectrum correlations in nucleic acids. I. Raman lines in the 600-700 cm-1 range of guanosine residue.
• Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety.
• Immunosuppression by human plasma alpha 1-acid glycoprotein: importance of the carbohydrate moiety.
• Triple helices containing arabinonucleotides in the third (Hoogsteen) strand: effects of inverted stereochemistry at the 2'-position of the sugar moiety.