Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.
AUTOR(ES)
Briglia, M
RESUMO
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167922Documentos Relacionados
- Detection of Methylobacterium species by 16S rRNA gene-targeted PCR.
- Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution
- Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces
- Selective and Sensitive Method for PCR Amplification of Escherichia coli 16S rRNA Genes in Soil
- Rapid detection and identification of Mycobacterium avium by amplification of 16S rRNA sequences.
