Rapid Detection and Identification of Respiratory Viruses by Direct Immunofluorescence
AUTOR(ES)
D'Alessio, Donn
RESUMO
The use of fluorescein-conjugated antiserum against respiratory syncytial (RS) and parainfluenza 1 and 3 viruses was compared with conventional techniques in the rapid detection of virus in tissue cultures inoculated with pharyngeal specimens known to contain these viruses. Twenty-three specimens were tested: 9 RS, 8 parainfluenza 1, and 6 parainfluenza 3. The fluorescent-antibody technique (FA) detected virus in 52% of the tissue cultures in 24 hr, and, by 72 hr, 22 of the 23 cultures were FA-positive whereas only 5 were positive by conventional techniques. Additionally, conjugated antisera were prepared against herpes simplex, influenza A2, and adenovirus type 5. All conjugates stained only the homologous virus and were 100- to 10,000-fold more sensitive than conventional techniques in detecting descending dilutions of virus inocula by 24 hr. With the procedures described, several antisera could be conjugated and ready for use within 24 hr. Serum fractionation was by ammonium sulfate precipitation, and with the procedure outlined virtually complete recovery of the globulin fraction and elimination of all of the albumin were accomplished.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376907Documentos Relacionados
- Efficiency of immunofluorescence for rapid detection of common respiratory viruses.
- Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses
- SimulFluor Respiratory Screen for Rapid Detection of Multiple Respiratory Viruses in Clinical Specimens by Immunofluorescence Staining
- Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies.
- Rapid detection and identification of Bacteroides fragilis and Bacteroides melaninogenicus by immunofluorescence.