Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.
AUTOR(ES)
Delisle, G J
RESUMO
A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to 90%, and the specificity was 100%.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267418Documentos Relacionados
- Efficacy of beta-glucuronidase assay for identification of Escherichia coli by the defined-substrate technology.
- Detection of active heteropolymeric beta-glucuronidase in hybrids between mouse cells and human fibroblasts with beta-glucuronidase deficiency.
- beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
- Expression of the Escherichia coli beta-glucuronidase gene in Pseudocercosporella herpotrichoides.
- Colorimetric enumeration of Escherichia coli based on beta-glucuronidase activity.
