Rapid estimation of serum cholinesterase activity using the Astrup micro equipment
AUTOR(ES)
Johnson, J. K.
RESUMO
A rapid micro technique for the estimation of serum cholinesterase is described. Acetylcholine bromide is incubated with serum within the capillary of the Astrup electrode. The enzyme hydrolyses the substrate with the liberation of acetic acid. This causes a fall of pH which is seen on the galvanometer of the instrument and the rate of this fall is shown to be proportional to enzyme concentration.
