Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.
AUTOR(ES)
Saunders, G C
RESUMO
We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=274363Documentos Relacionados
- Use of enzyme-labeled antibodies to detect Salmonella in foods.
- Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.
- Development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene.
- Detection of specific immunoglobulin M antibody to rubella virus by use of an enzyme-labeled antigen.
- Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen.