Rapid parallel mutation scanning of gene fragments using a microelectronic protein–DNA chip format

AUTOR(ES)

Behrensdorf, Heike A.

FONTE

Oxford University Press

RESUMO

We have developed a method for the de novo discovery of genetic variations, including single nucleotide polymorphisms and mutations, on microelectronic chip devices. The method combines the features of electronically controlled DNA hybridisation on open-format microarrays, with mutation detection by a fluorescence-labelled mismatch- binding protein. Electronic addressing of DNA strands to distinct test sites of the chip allows parallel analysis of several individuals, as demonstrated for mutations in different exons of the p53 gene. This microelectronic chip-based mutation discovery assay may substitute for time-consuming sequencing studies and will complement existing technologies in genomic research.

Documentos Relacionados

• Thermodynamics of Cro protein-DNA interactions.
• Temperature-sensitive protein–DNA dimerizers
• Protein-DNA interactions upstream from the human A gamma globin gene.
• A permutational approach toward protein-DNA recognition.
• Quantitative evaluation of protein–DNA interactions using an optimized knowledge-based potential