Rapid Pulsed-Field Gel Electrophoresis Method for Group B Streptococcus Isolates
AUTOR(ES)
Benson, Jeffrey A.
FONTE
American Society for Microbiology
RESUMO
We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=88282Documentos Relacionados
- Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis.
- Macrorestriction Fingerprinting of “Streptococcus milleri” Group Bacteria by Pulsed-Field Gel Electrophoresis
- Rapid method for preparation and cleavage of bacterial DNA for pulsed-field gel electrophoresis.
- Partitioned pulsed-field gel electrophoresis-PCR (PPF-PCR): a new method for pulsed-field mapping for STS and microsatellites.
- Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates.