Rapid Real-Time PCR for Determination of Penicillin Susceptibility in Pneumococcal Meningitis, Including Culture-Negative Cases
AUTOR(ES)
Kearns, Angela M.
FONTE
American Society for Microbiology
RESUMO
A novel real-time PCR-hybridization assay was developed for the rapid (<1 h) detection of penicillin susceptibility in Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive S. pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=153399Documentos Relacionados
- Rapid and Accurate Identification of Coagulase-Negative Staphylococci by Real-Time PCR
- Application of Real-Time PCR for Determination of Antiviral Drug Susceptibility of Herpes Simplex Virus
- Prediction of Decreased Susceptibility to Penicillin of Neisseria meningitidis Strains by Real-Time PCR
- Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR
- Culture-Negative Neonatal Meningitis and Endocarditis Caused by Streptococcus agalactiae