Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
AUTOR(ES)
Bina-Stein, M
RESUMO
A method for the determination of the primary structure of spliced mRNA junction and leader sequences is described. By analogy to the DNA sequencing procedure of Sanger et al. [Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci USA 74, 5463--5467], we use 2",3'-dideoxynucleoside triphosphates as chain-terminating inhibitors of the reverse transcriptase (RNA-dependent DNA polymerase) reaction. By using specific DNA restriction fragments as primers in combination with this technique, we have determined the sequence of the spliced junction between the body and the leader sequence of the 16S late mRNA of simian virus 40. The method described should be of general utility in mapping spliced mRNA regions for which the corresponding protein sequence (if any) is unknown.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=383033Documentos Relacionados
- The number of ribosomes on simian virus 40 late 16S mRNA is determined in part by the nucleotide sequence of its leader.
- Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.
- Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.
- Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40.
- Sequence heterogeneity at the 5' termini of late simian virus 40 19S and 16S mRNAs.
