Rat prostatic steroid binding protein: DNA sequence and transcript maps of the two C3 genes.
AUTOR(ES)
Hurst, H C
RESUMO
In the rat there are two non-allelic genes C3(1) and C3(2) for the C3 polypeptide of prostatic steroid binding protein. We have cloned and sequenced both genes and show that only C3(1) is responsible for the production of authentic C3. Although there is a marked difference in their transcriptional activity, the two genes share extensive DNA sequence homology there being only one base difference from nucleotide - 235 to within the first intron. Transcript mapping has shown that there are two distinct C3 transcripts which share a unique 3' terminus but have 5' termini 38 bases apart each preceded by a 'TATA' box homology. Interestingly, an identical repetitive element is present just upstream of both genes. Both families of transcripts, which are produced in a ratio of 18:1, are coordinately regulated by testosterone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=555183Documentos Relacionados
- Rat prostatic steroid binding protein: characterisation of the Alu element upstream of the C3 genes.
- Prostatic steroid binding protein: organisation of C1 and C2 genes.
- Rat prostatic binding protein: the complete sequence of the C2 gene and its flanking regions.
- The miniF plasmid C protein: sequence, purification and DNA binding.
- Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA.
