Rate maintenance of cell division in Escherichia coli B/r: analysis of a simple nutritional shift-down.
AUTOR(ES)
Zaritsky, A
RESUMO
A competitive (nonmetabolizable) inhibitor of glucose uptake, alpha-methylglucoside, was used to limit the growth of Escherichia coli. Cell division during such a nutritional shift-down was studied in batch cultures and with the "baby-machine" technique. Following a brief delay, the rate of division was maintained for 60 to 70 min in batch cultures and for an extended period in the baby machine. Decreases in cell size were due, in part, to a possible reduction in the mass per chromosome origin at the time of replication initiation and a shorter time interval between initiation and the subsequent division. These unusual findings suggest that this method for abrupt change in growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207556Documentos Relacionados
- Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein.
- Control of protein synthesis in Escherichia coli: strain differences in control of translational initiation after energy source shift-down.
- Control of protein synthesis in Escherichia coli: control of bacteriophage Q beta coat protein synthesis after energy source shift-down.
- Genetic Analysis of the Leucine Region in Escherichia coli B/r: Gene-Enzyme Assignments
- Control of Ribosome Synthesis in Escherichia coli: Analysis of an Energy Source Shift-Down
