Razão entre o conteúdo extracelular e intracelular de HSP70 como um sinal de alerta imunológico e marcador de intensidade de exercício

AUTOR(ES)

Thiago Gomes Heck

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

2011

RESUMO

Living organisms respond to physiological challenges, such as heat shock treatment and/or exercise with a rapid and marked increase in some of some specific gene expression: The heat-shock response that occurs in many cells inducing the 70-kDa Heat-Shock Protein (HSP70) synthesis. HSP70 has antiinflammatory cytoprotective functions when located at the intracellular environment. Once HSP70 can be released to extracellular environment, the extracellular (eHSP70) plays pro-inflammatory functions. This study evaluated the effects of heat shock in lymphocytes from mesenteric lymph nodes taken from rats previously subjected to different intensities of swimming exercise. Both exercise and heat shock were able to induce the HSP70 synthesis in lymphocytes, and increased cellular content of HSP70 and the translocation of HSP70 to the nuclear region was associated with the inactivation of NF-kB, as observed in the maintenance of this pro-inflammatory transcription factor in the cytoplasm, that may be related to cell proliferation decrease. Although this effect of exercise on HSP70 expression was related with exercise intensity, this study showed that there exist a threshold for exercise-induced HSP70 expression, since higher exercise loads were not ableto increase HSP70 synthesis further in lymphocytes. Additionally, high-intensity exercise was associated to eHSP70 increase and enhanced intra-toextracellular HSP70 ratio in lymphocytes. The eHSP70/HSP70 ratio also was also increased in response to heat shock, but higher levels was observed if the rat was submitted to previous high-intensity exercise session. These data suggest that HSP70 may be a new exercise intensity marker, primarily using immune cells because of the possibility of this cells use HSP70 as a danger signal to the whole body.

ASSUNTO(S)

exercício fisiologia do exercício

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