Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy
AUTOR(ES)
Schroeder, Marie A.
FONTE
The Federation of American Societies for Experimental Biology
RESUMO
The Krebs cycle plays a fundamental role in cardiac energy production and is often implicated in the energetic imbalance characteristic of heart disease. In this study, we measured Krebs cycle flux in real time in perfused rat hearts using hyperpolarized magnetic resonance spectroscopy (MRS). [2-13C]Pyruvate was hyperpolarized and infused into isolated perfused hearts in both healthy and postischemic metabolic states. We followed the enzymatic conversion of pyruvate to lactate, acetylcarnitine, citrate, and glutamate with 1 s temporal resolution. The appearance of 13C-labeled glutamate was delayed compared with that of other metabolites, indicating that Krebs cycle flux can be measured directly. The production of 13C-labeled citrate and glutamate was decreased postischemia, as opposed to lactate, which was significantly elevated. These results showed that the control and fluxes of the Krebs cycle in heart disease can be studied using hyperpolarized [2-13C]pyruvate.—Schroeder, M. A., Atherton, H. J., Ball, D. R., Cole, M. A., Heather, L. C., Griffin, J. L., Clarke, K., Radda, G. K. Tyler, D. J. Real-time assessment of Krebs cycle metabolism using hyperpolarized 13C magnetic resonance spectroscopy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2717776Documentos Relacionados
- NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY: 13C SPECTRA OF UNSUBSTITUTED INOSITOLS*
- Pyruvate metabolism in Halobacterium salinarium studied by intracellular 13C nuclear magnetic resonance spectroscopy.
- Nuclear Magnetic Resonance Spectroscopy: 13C Spectra of Some Common Nucleotides*
- 13C Nuclear Magnetic Resonance Spectroscopy of Native and Recombined Lipoproteins
- Quantitative Assessment of Lung Using Hyperpolarized Magnetic Resonance Imaging
