Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens

AUTOR(ES)

Aigner, Cleiton Paulo, Silva, Aristeu Vieira da, Sandrini, Fabiano, Osório, Paulo de Sá, Poiares, Lilian, Largura, Alvaro

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2010-11

RESUMO

This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies. Brain and heart samples were collected from 26 seropositive chickens and DNA was extracted using Trizol® and amplified using real-time PCR with SYBR® Green. Parasite DNA was detected in 24 of the 26 samples analyzed; the number of positive tissue samples and the parasite quantity did not differ between tissue types. The results confirmed the analytical sensitivity of parasite detection in chicken tissue samples and demonstrated the possibility of using other molecular systems for genotypic analysis.

Documentos Relacionados

• Real-time PCR-based method for the estimation of genome sizes
• Real-Time PCR-Based System for Simultaneous Quantification of Human Papillomavirus Types Associated with High Risk of Cervical Cancer
• Real-Time PCR for Quantitative Detection of Toxoplasma gondii
• Development and Evaluation of Real-Time PCR-Based Fluorescence Assays for Detection of Chlamydia pneumoniae
• PCR-Based Diagnosis of Helicobacter pylori Infection and Real-Time Determination of Clarithromycin Resistance Directly from Human Gastric Biopsy Samples