Reassociation Kinetics for Epstein-Barr Virus DNA: Nonhomology to Mammalian DNA and Homology of Viral DNA in Various Diseases
AUTOR(ES)
Kawai, Yasuo
RESUMO
The number of Epstein-Barr virus (EBV) genomes per cell in established leukocytic lines and tissue specimens has been evaluated by measuring DNA-DNA reassociation kinetics with hydroxyapatite chromatography. Under the proper conditions, this method is sufficiently sensitive to detect EBV DNA in the amount of 0.1 genome per cell. All the samples tested that have been suspected to be without EBV DNA by cRNA hybridization proved negative by this more sensitive specific analysis. These included Hela and Hep2 cells, a negative case of Burkitt's lymphoma, two negative cases of nasopharyngeal carcinoma, and two established human leukocytic lines. Homology tests conducted with single-strand-specific nuclease S1 indicated that the viral DNA from a nasopharyngeal carcinoma and infectious mononucleosis were more than 90% homologous to EBV DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356729Documentos Relacionados
- DNA of Epstein-Barr virus. V. Direct repeats of the ends of Epstein-Barr virus DNA.
- Improved immunofluorescence antigens for detection of immunoglobulin M antibodies to Epstein-Barr viral capsid antigen and antibodies to Epstein-Barr virus nuclear antigen.
- Effects of S-adenosylhomocysteine and analogs on Epstein-Barr virus-induced transformation, expression of the Epstein-Barr virus capsid antigen, and methylation of Epstein-Barr virus DNA.
- Immune regulation in Epstein-Barr virus-associated diseases.
- Defective viral DNA in Epstein-Barr virus-associated oral hairy leukoplakia.
