Reatividade antigênica entre Phytomonas serpens E Trypanosoma cruzi : uma análise proteômica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species and are responsible for important phytopatologies resulting in great economic losses. Phytomonas serpens, a trypanosomatid that parasitize tomato, is transmitted among plants by phytofagous insects. Previous studies in our laboratory showed that P. serpens presents an antigenic reactivity with Trypanosoma cruzi, the etiologic agent of Chagas´ disease. Sera of patients with Chagas? disease reacted with total protein extract from P. serpens, in Western blotting assays. In addiction, oral immunization with live promastigotes of P. serpens reduced significantly the parasitemia and mortality in mice challenged with a high number of tripomastigotes of T. cruzi. In this work was realized a proteomic analysis of P. serpens 15T, showing what proteins reacted with sera of Chagasic patients. After 2D-electrophoresis of whole-cell lysates of P. serpens, 31 peptides were selected and analyzed by tandem mass spectrometry (MS/MS). Twenty eight peptides were identified resulting in 22 different putative proteins. The majority of this polypeptides are involved in parasite metabolism. It was also showed that lymph node cells isolated of BALB/c mice previously immunized with P. serpens present significant stimulation indices in the presence of total antigens of T. cruzi. The activity of ethanolic extract of Erythrina speciosa (Et-Es) over T. cruzi and P. serpens was also analysed in this study. The anti-proliferative activity of Et-Es against epimastigote forms of T. cruzi culture was observed in the concentrations of 250, 500 e 1000 ug/mL, where it was observed a growth inhibition of 50, 60 e 75% respectively. However, the administration of Et-Es (50mg/kg body weight) in Swiss mice infected with 5x103 tripomastigotes of T. cruzi, resulted in a significant increase in parasitemia when compared to control group (infected and non- treated). In P. serpens, it was possible to observe an anti-proliferative activity dose-dependent of Et-Es over promastigotes. In concentration of 1000 ug/mL it was observed a growth inhibition of 100%.

ASSUNTO(S)

phytomonas serpens microbiology trypanosoma cruzi microbiologia tripanossoma cruzi

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