RecA-independent recombination at gamma delta termini and at IS3 producing inverted repetition in F' plasmids.
AUTOR(ES)
Tsai, M
RESUMO
Two F' plasmids isolated independently from a recA- strain of Escherichia coli and containing identical deletion end points and identical associated inverted duplications are described. In these plasmids, DNA of F plasmid from the IS3 element alpha 1 beta 1 up to the transposon gamma delta is duplicated in inverted orientation, and a 63-kilobase-pair segment from the chromosomal DNA of the plasmid is deleted. One deletion terminus is the chromosomal IS3 alpha 4 beta 3 carried by the parental plasmid, ORF203. It is proposed that these structures resulted from interduplex strand exchanges that occurred at the ends of the movable element gamma delta and at IS3. This indicates that the 35-base-pair gamma delta termini can participate in genome rearrangements by mechanisms that are distinct from complete transposition mechanisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391593Documentos Relacionados
- recA-dependent recombination between rRNA operons generates type II F' plasmids.
- Role of R loops in recA-independent homologous recombination of bacteriophage lambda.
- recA-independent recombination between repeated IS50 elements is not caused by an IS50-encoded function.
- Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.
- RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli