Recombinant hepatitis E virus genomes infectious for primates: Importance of capping and discovery of a cis-reactive element
AUTOR(ES)
Emerson, S. U.
FONTE
The National Academy of Sciences
RESUMO
Hepatitis E virus recombinant genomes transcribed in vitro from two cDNA clones differing by two nucleotides were infectious for chimpanzees. However, one cDNA clone encoded a virus that was attenuated for chimpanzees and unable to infect rhesus monkeys. The second cDNA clone encoded a virus that infected both chimpanzees and rhesus monkeys and caused acute hepatitis in both. One mutation differentiating the two clones identified a cis-reactive element that appeared to overlap the 3′ end of the capsid gene and part of the 3′ noncoding region. Capping of the RNA transcripts was essential for infectivity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=65019Documentos Relacionados
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