Recombination between bacteriophage lambda and plasmid pBR322 in Escherichia coli.
AUTOR(ES)
Pogue-Geile, K L
RESUMO
Recombinant lambda phages were isolated that resulted from recombination between the lambda genome and plasmid pBR322 in Escherichia coli, even though these deoxyribonucleic acids (DNAs) did not share extensive regions of homology. The characterization of these recombinant DNAs by heteroduplex analysis and restriction endonucleases is described. All but one of the recombinants appeared to have resulted from reciprocal recombination between a site on lambda DNA and a site on the plasmid. In general, there were two classes of recombinants. One class appeared to have resulted from recombination at the phage attachment site that probably resulted from lambda integration into secondary attachment sites on the plasmid. Seven different secondary attachment sites on pBR322 were found. The other class resulted from plasmid integration at other sites that were widely scattered on the lambda genome. For this second class of recombinants, more than one site on the plasmid could recombine with lambda DNA. Thus, the recombination did not appear to be site specific with respect to lambda or the plasmid. Possible mechanisms for generating these recombinants are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=294126Documentos Relacionados
- Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli.
- Conservative integration of bacteriophage Mu DNA into pBR322 plasmid.
- Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication.
- Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMG110 from Escherichia coli.
- Effect of nalidixic acid and novobiocin on pBR322 genetic expression in Escherichia coli minicells.