Reconstitution of Colicin E2-Induced Deoxyribonucleic Acid Degradation in Spheroplast Preparations
AUTOR(ES)
Almendinger, R.
RESUMO
Spheroplasts are insensitive to colicin E2 and do not show deoxyribonucleic acid (DNA) degradation even in the presence of massive amounts of E2. However, when both endonuclease I and E2 were present, spheroplast DNA was degraded by an endonucleolytic activity which gave rise primarily to double-strand DNA cleavages, producing fragments having an average molecular weight of 9 × 106. Pancreatic ribonuclease could substitute for colicin E2 in the reconstitution system, but pancreatic deoxyribonuclease could not replace endonuclease I. However, colicin E2 could not activate transfer ribonucleic acid-inhibited endonuclease I in an in vitro system where pancreatic ribonuclease caused full stimulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444523Documentos Relacionados
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