Reduced Delayed-Rectifier K+ Current in the Learning Mutant rutabaga
AUTOR(ES)
Alshuaib, Waleed B.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
In the Drosophila mutant rutabaga, short-term memory is deficient and intracellular cyclic adenosine monophosphate (cAMP) concentration is reduced. We characterized the delayed-rectifier potassium current (IKDR) in rutabaga as compared with the wild-type. The conventional whole-cell patch-clamp technique was applied to cultured Drosophila neurons derived from embryonic neuroblasts. IKDR was smaller in rutabaga (368 ± 11 pA) than in wild-type (541 ± 14 pA) neurons, measured in a Ca2+-free solution. IKDR was clearly activated at ∼0 mV in the two genotypes. IKDR typically reached its peak within 10–20 msec after the start of the pulse (60 mV). There was no difference in inactivation of IKDR for wild-type (14 ± 3%) and rutabaga (19 ± 3%). After application of 10 mM TEA, in wild-type, IKDR was reduced by 46 ± 5%, whereas in rutabaga, IKDR was reduced by 28 ± 3%. Our results suggest that IKDR is carried by two different types of channels, one which is TEA-sensitive, whereas the other is TEA-insensitive. Apparently, the TEA-sensitive channel is less expressed in rutabaga neurons than in wild-type neurons. Conceivably, altered neuronal excitability in the rutabaga mutant could disrupt the processing of neural signals necessary for learning and memory.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=187589Documentos Relacionados
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