Reduction of interfering cytotoxicity associated with wastewater sludge concentrates assayed for indigenous enteric viruses.
AUTOR(ES)
Hurst, C J
RESUMO
Washing, freon extraction, and cationic polyelectrolyte precipitation were compared for their ability to reduce cytotoxicity associated with virus concentrates derived from beef extract eluates of wastewater sludges. Eluates concentrated by hydroextraction were usually much more toxic than those concentrated by organic flocculation. This difference may be due entirely to nondialyzable material naturally present in the beef extract which did not precipitate during flocculation at pH 3.5. Washing inoculated cell monolayers with saline containing calf serum before the addition of agar overlay media was most effective in reducing cytotoxicity, although it resulted in a greater virus loss, as compared with freon extraction and cationic polyelectrolyte precipitation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239278Documentos Relacionados
- Comparison of methods for recovering indigenous viruses from raw wastewater sludge.
- Heat inactivation of enteric viruses in dewatered wastewater sludge.
- Inactivation of enteric viruses in wastewater sludge through dewatering by evaporation.
- Reduction of Cytotoxicity in Virus Concentrates from Environmental Samples
- Simultaneous infections with different enteric and respiratory tract viruses.