REDUCTION OF SELENITE BY INTACT YEAST CELLS AND CELL-FREE PREPARATIONS
AUTOR(ES)
Falcone, Giuseppe
RESUMO
Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754–762. 1963.—Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absence of added substrate. Cell suspensions reduced selenite optimally at pH 4.2. No added metabolite was found to be stimulatory; reduction was inhibited by methionine and formate as well as by fluoride, dinitrophenol, and certain sulfhydryl poisons. Cell-free preparations capable of reducing selenite were obtained from C. albicans and from baker's yeast disintegrated in a Hughes press. The enzymatic system had optimal activity at pH 7 with 10−2m selenite. Activity of the system was lost on dialysis but was restored upon the addition of dialyzable substances or of boiled, undialyzed extract.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=278222Documentos Relacionados
- METABOLISM OF CARBOHYDRATES BY PSEUDOMONAS SACCHAROPHILA I. : Oxidation of Fructose by Intact Cells and Crude Cell-Free Preparations1
- NITROGEN FIXATION BY CELL-FREE PREPARATIONS FROM MICROORGANISMS*
- Degradation of lindane by cell-free preparations of Clostridium sphenoides.
- Nuclear Binding of Steroid Receptors: Comparison in Intact Cells and Cell-Free Systems
- POLYSACCHARIDE AND GLYCOLIPID SYNTHESIS BY CELL-FREE PREPARATIONS FROM TYPE XIV PNEUMOCOCCUS*